3.12-200ng/ml

1.21ng/ml

This assay has high sensitivity and excellent specificity for detection of Anti-Collagen Type I Antibody (Anti-COL1). No significant cross-reactivity or interference between Anti-Collagen Type I Antibody (Anti-COL1) and analogues was observed.

Intra-Assay %CV: <10% Inter-Assay %CV: <12%

The microtiter plate provided in this kit has been pre-coated with COL1. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-COL1 will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-COL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Pre-coated, ready to use 96-well strip plate Standard, 2x vials Detection Reagent A, 1x120ul Detection Reagent B, 1x120ul TMB Substrate, 1x9ml Wash Buffer (30X): 1x20ml Standard Diluent, 1x20ml Assay Diluent A, 1x12ml Assay Diluent B, 1x12ml Stop Solution, 1x6ml

Store Microtiter Strips, Standard, Detection Reagents and Positive Control at -20°C. After reconstitution of Positive Control, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.