Patients with debilitated immune systems that spend long periods of time in hospitals and other healthcare centres are especially susceptible to C.auris infections. The fungus can spread through contact with contaminated surfaces or from person to person. Treatment options are limited because C.auris shows reduced sensibility to azoles, polyenes, and echinocandins, and some strains are resistant to all three types of drugs. Infections can affect the bloodstream, central nervous system, kidneys, liver, bones, muscles, joints, spleen or eyes. High mortality rates have been associated with a delay in the initiation of the appropriate treatment, meaning that rapid identification from patient samples is key to administrate the right treatment and implement appropriate control measures to avoid the spread of the infection.

Phenotypic methods commercially available often misidentify C.auris as C. haemulonii (Candida species phylogenetically related, also commonly found in hospital settings), but also as other yeast species including C. famata, C. guilliermondii, C. lusitaniae, C. parapsilosis, C. sake, Rhodotorula glutinis, and Saccharomyces cerevisiae). Other methods, like matrix- assisted laser or sequencing of the internal transcribed spacer (ITS) and D1/D2 of the ribosomal gene have been shown to accurately detect C.auris. Although these are high quality techniques to successfully differentiate C.auris, they are time consuming. qPCR offers a reliable method to rapidly identify infected or colonized patients, which is crucial for the prompt administration of the right treatment and implementing effective infection control measures.

The genesig® Kit Candida auris (C.auris) genomes are designed for the in vitro quantification of C.auris genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

Leach L, Zhu Y, Chaturvedi S. 2018. Development and validation of a real-time PCR assay for rapid detection of Candida auris from surveillance samples. J Clin Microbiol 56:e01223 -17. This assay displays possible in-situ cross-reaction with Candida haemulonis

• Exceptional value for money • Rapid detection of all clinically relevant subtypes • Positive copy no. standard curve for quantification • Highly specific detection profile • High priming efficiency • Broad dynamic detection range (>6 logs) • Sensitive to < 100 copies of target • Accurate controls to confirm findings

All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration, and DNA integrity (An internal PCR control is supplied to test for non specific PCR inhibitors). Always run at least one negative control with the samples. To prepare a negative-control, replace the template DNA sample with RNase/DNase free water.

Under optimal PCR conditions genesig C.auris detection kits have very high priming efficiencies of >95% and can detect less than 100 copies of target template.

A C.auris specific primer and probe mix is provided and this can be detected through the FAM channel. The primer and probe mix provided exploits the so-called TaqMan® principle. During PCR amplification, forward and reverse primers hybridize to the C.auris DNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`-dye and a 3`-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. The resulting increase in fluorescence can be detected on a range of qPCR platforms.

• C.auris specific primer/probe mix (150 reactions BROWN) FAM labelled • C.auris positive control template (for Standard Curve (RED) • Internal extraction control primer/probe mix (150 reactions BROWN) VIC labelled as standard • Internal extraction control DNA (150 reactions BLUE) • Endogenous control primer/probe mix (150 reactions BROWN) FAM labelled • RNase/DNase free water, for resuspension of primer/probe mixes (WHITE) • Template preparation buffer, for resuspension of internal control template, positive control template and standard curve preparation (YELLOW)

• Real-Time PCR Instrument • DNA extraction kit This kit is recommended for use with genesig Easy. DNA/RNA Extraction Kit. However, it is designed to work well with all processes that yield high quality DNA with minimal PCR inhibitors. • oasigTM lyophilized or Precision Plus 2X qPCR Master Mix. This kit is intended for use with oasig or PrecisionPLUS 2X qPCR Master Mix. • Pipettors and Tips • Vortex and centrifuge • Thin walled 1.5 ml PCR reaction tubes

This kit is stable at room temperature but should be stored at -20ºC on arrival. Once the lyophilized components have been resuspended they should not be exposed to temperatures above -20ºC for longer than 30 minutes at a time and unnecessary repeated freeze/thawing should be avoided. The kit is stable for six months from the date of resuspension under these circumstances.

genesig® kits are sold for research use only and are not licensed for diagnostic procedures.

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative Real-Time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented Real-Time PCR expertise in design and validation ensure the best possible kit. 

genesig® Easy DNA/RNA extraction kits (sold separately) are a simple to use, low cost and powerful extraction technology suitable for virtually all sample types.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

 All products should be handled by qualified personnel only, trained in laboratory procedures.