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C2088-60P Caspase 12, NT, Blocking Peptide

Specifications
References
Purity
Purified
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

Peptide (AARRTHERDPIYKIKG) corresponding to aa2-17 of murine caspase-12 (3).

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is colocalized to the ER with several proteins that are involved in Alzheimer’s disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues (1).
Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.
Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Synthetic peptide
Purity
Purified
Concentration
~0.2mg/ml
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98–103.||2. Mehmet H. Caspases find a new place to hide. Nature 2000;403:29–30||3. Van de Craen M, Vandenabeele P, Declercq W, Van den Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W, Beyaert R, Fiers W. Characterization of seven murine caspase family members. FEBS Lett 1997;403:61–9
USBio References
No references available
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