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C2555-01-APC cdc14A Phosphatase (APC)

Specifications
Conjugate
APC
Specificity
Recognizes the ~80kD human Cdc14A protein. Reactivity is confirmed with human HEK293 cell lysates.
Source Antibody
human
Purity
Purified by immunoaffinity chromatography.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
BSA Free

Cdc14, also known as duel specificity phosphatase, is a protein phosphatase originally discovered in yeast that plays an important role in cell cycle regulation, including exit from mitosis. Throughout G1, S and G2 phases, Cdc14 is located in the nucleolus; at the beginning of anaphase, the protein is redistributed throughout the entire nucleus. In late anaphase, Cdc14 dephosphorylates Cdh1 which then activates APC (Anaphase Promoting Complex). Two human orthologs of yeast Cdc14 have been identified; these are Cdc14A and Cdc14B, and they share about 80% amino acid homology in the N-termini and phosphatase domains. Cdc14 has also been shown to dephosphorylate p53, suggesting that it may play a role in regulation of p53 activity.

Applications
Suitable for use in Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunofluorescence: 1-5ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Pricing
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