One kit of this lot has been tested to go through the complete compartmental protein extraction procedure from rat colon. Four compartmental proteins from the extraction are used for electrophoresis, transferring to PVDF membrane and immunoblotting with mouse anti-GAPDH, anti-histone H1, anti-vimentin, and Na/K+ ATPase as primary antibodies, and HRP-conjugated anti-mouse IgG as secondary antibody. The separation of four compartment proteins is confirmed by the enrichment of GAPDH in the cytoplasmic fraction, Histone H1 in the nuclear fraction, Na/K+ ATPase in the membrane fraction, and the unique localization of Vimentin in the cytoskeleton fraction.

C6012-25A: Buffer C: HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium orthovanadate, 1x18ml C6012-25B: Buffer CS: Pipes, pH 6.8, MgCl2, sodium chloride, EDTA, sucrose, SDS, sodium orthovanadate, 1x3ml C6012-25C: Buffer M: HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40, sodium orthovanadate, 1x6ml C6012-25D: Buffer N: HEPES, pH 7.9, MgCl2, sodium chloride, EDTA, glycerol, sodium orthovanadate, 1x6ml C6012-25E: Buffer W: HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium orthovanadate, 1x50ml C6012-25F: Protease Inhibitors (50X): A cocktail of protease inhibitors, 1x660ul

Reagents are sufficient for extraction of compartment proteins from 5g tissue or about 1.25x10e6 cells. Minimal 0.1g tissue or 2.5x10e6 cells are required.

Aliquot and store *C6012-25F at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.