The Human Complement C3 ELISA Kit is an in vitro immunoassay for the quantification of C3 in serum of other biological fluids. The kit performance has been optimized for the protocol and materials listed below using standard dilutions of human Complement C3 in the 0.5-500ng/ml range. The operator must determine appropriate dilutions of reagents for alternative assay conditions. ELISA assay reactivity is sensitive to variations in operator, pipetting and washing techniques, incubation time, temperature, composition of reagents, and other experimental variables. Assay optimization may be required to generate the standard curve and fit the samples in the specified detection range.
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