The blood cells in whole blood or other cultured cells and serum are first lysed in the lysis binding buffer that contains the denaturant guanidine HCl and Triton X100. Ethanol is then added to the samples, which are then added to the filter spin columns. This step facilitates the binding of DNA to the filter matrix. Under these conditions the DNA binds to the membrane while other contaminants are washed through. The columns are then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final genomic DNA product is eluted in TE. The final DNA product can be used directly for quantitative PCR and other downstream applications.

3ug-9ug

UV260/280> 1.7

99% of DNA is > 20kb

Total time of prep less than 25 min

Item Amount Storage 1. Lysis Binding Buffer 21ml RT

2. Wash Buffer 1 26ml RT

3. Wash Buffer 2 12ml RT

4. TE 10ml RT

5. spin column set 100 units RT