Supplied with 6x Loading Dye Solution
pBR322 DNA was completely digested by BsuRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 22 discrete fragments (in base pairs): 587, 540, 502, 458, 434, 267, 234, 213, 192, 184, 124, 123, 104, 89, 80, 64, 57, 51, 21, 18, 11, 8.
Concentration
0.5mg DNA/ml
Storage Buffer
10mM Tris-HCl (pH 7.6), 1mM EDTA
6X Loading Dye Solution
0.09% bromophenol blue, 0.09% xylene cyanol, 60% glycerol, 60mM EDTA.
Fragment (bp) % ng DNA 587 13.4 134 540 12.4 124 504 11.6 116 458 10.5 105 434 10.0 100 267 6.1 61 234 5.3 53 213 4.9 49 192 4.4 44 184 4.2 42 124 2.8 28 123 2.8 28 104 2.4 24 89 2.0 20 80 1.8 18 64 1.5 15 57 1.3 13 51 1.2 12 21 0.5 5 18 0.4 4 11 0.3 3 8 0.2 2
Agarose Gel Loading
DNA Marker Labeling Protocols 1. Prepare the following reaction mixture 1ul (0.5ug) DNA Marker 1ul of 6x Loading Dye solution 4ul Deionized Water 2. Vortex gently prior to use. Do not heat before loading 3. Apply 0.2ul (0.1ug) of DNA marker per 1mm of agarose gel lane width.
Following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining.
Polyacrylamide Gel Loading
1. Prepare the following reaction mixture 2ul (1ug) DNA Marker 0.5ul of 6x Loading Dye solution 0.5ul Deionized Water 2. Vortex gently prior to use. Do not heat before loading 3. Apply 0.4ul (0.2ug) of DNA marker per 1mm of polyacrylamide gel lane width.
Following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining.
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