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E3452-61A-ML650 ERK5, phosphorylated (Thr218, Tyr220) (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7) (MaxLight 650)

Specifications
References
Brand
MaxLight™
Conjugate
MaxLight™650
Specificity
Recognizes human ERK5, phosphorylated at Thr218 and Tyr220, Mr ~115kD.
Source Antibody
human
Purity
Purified by affinity chromatography.
Accession Number
NM_139033
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
EC=2.7.11.24

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Note: Basal levels of phosphorylated ERK5 in non-stimulated HeLa cells can be eliminated by treatment with Lambda Protein Phosphatase. The calculated Molecular Weight: is 88kD but is observed at ~115kD. Unidetified bands may appear in some lysates.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Zhou, G., et al., (1995) J. Biol. Chem. 270:12665-12669.
USBio References
No references available
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