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G3059-11 β-Glucosidase Assay Kit, BioAssay™

Specifications
References
Brand
BioAssay™
Kit Type
Assay
Tests
96
Sample Volume
20ul
Detection Method
Colorimetric/Fluorescent
Detection Range
The detection limit is 2U/L, linear up to 250U/L.
EU Commodity Code
38220000
Shipping Temp
RT
Storage Temp
-20°C

b-Glucosidase is a glucosidase enzyme which acts upon beta1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). b-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a b-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a b-glucosidase isoform (lysosomal glucocerebrosidase) has been related to Gaucher's disease and Parkinson's disease. This b-Glucosidase Assay Kit is designed to measure b-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-b-D-glucopyranoside that is hydrolyzed specifically by b-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

Key Features
High sensitivity and wide linear range. Use 20ul sample. The detection limit is 2U/L, linear up to 250U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of b-glucosidase activity within 20 minutes. Robust and amenable to high throughput analysis.
Applications
Direct Assays: b-glucosidase activity in biological samples. Characterization and Quality Control for b-glucosidase production. Drug Discovery: high-throughput screen for b-glucosidase modulators.
Unit Definition
One unit of enzyme catalyzes the hydrolysis of 1mmole of substrate per min at pH 7.0.
Kit Components (100 tests in 96-well plates)
G3059-11A: Assay Buffer, pH 7.0, 1x24ml G3059-11B: b-NPG Substrate, 1x1ml G3059-11C: Calibrator, 1x10ml (equivalent to 250 U/L)
Storage and Stability
Store all components at -20°C. Stable for 6 months after receipt.
References
1. Bhat, M.K. et al. (1993). Purification and characterization of an extracellular β-glucosidase from the thermophilic fungus Sporotrichum thermophile and its influence on cellulase activity. Journal of General Microbiology 139: 2825-2832.|2. Kaur, J. et al (2007). Purification and characterization of β- glucosidase from Melanocarpus sp. Electronic J. Biotechn. 10: 260-270.|3. IW ASHIT A, K. et al., (1998) Purification and characterization of extracellular and cell wall bound β-glucosidases from Aspergillus kawachii. Bioscience, Biotechn. Biochem. 62(10): 1938-1946.
USBio References
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