USBio Logo

H5090-20A Histamine Receptor 3, NT, Control Peptide (HR3)

Specifications
References
Specificity
Synthetic peptide 18aa sequence within the extracellular, N-terminus of human HR3. The immunogen shows no significant sequence homology with other histamine receptors.|Species Sequence Homology: Mouse: 94%; rat: 88%; guinea pig: 84%.
Purity
Highly Purified
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

Control peptide for H5090-20 (affinity purified antibody) and H5090-20B (antiserum).

Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitter. It plays an important role in a multitude of physiological processes in central and peripheral tissues. Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g. sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation). In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells. Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system. Histamine effects are mediated by four pharmacologically distinct receptors: the H1R, H2R , H3R and H4R receptors. These receptors are all members of the G-protein coupled receptor (GPCR) family. Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length. Human Histamine 3 receptor, H3R (GPCR97), has recently been identified as a presynaptic autoreceptor on histamine neurons in the brain controlling the stimulated release of histamine. The H3 receptor has been shown to be a presynaptic heteroreceptor in nonhistamine- containing neurons in both the central and peripheral nervous systems. H3R (rat/human 445aa, ~93% homology) is primarily expressed in the brain. Several studies using H3 selective agonists revealed that H3R couples to pertussis toxin-sensitive Gi/o protein.
Applications
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to its low molecular weight. Other applications not tested.
Recommended Dilution
Antibody Blocking: 5-10ug per 1 ug H5090-20 (affinity purified antibody) or 1ul H5090-20B (antiserum). ELISA: 50-100ng control peptide per well. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Highly Purified
Concentration
~1mg/ml
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity
Synthetic peptide 18aa sequence within the extracellular, N-terminus of human HR3. The immunogen shows no significant sequence homology with other histamine receptors.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Lovenberg, T.W., et al., J. Pharmacol. Exp. Ther. 293: 771-778 (2000). 2. Lovenberg, T.W., et al., Mol. Pharmacol. 55: 1101-1107 (1999).
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved