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H5110-13J1 Histone H3, phosphorylated (Ser10) Blocking Peptide

Specifications
References
Specificity
Note: Adherence to the attached protocol is extremely important.
Purity
Purified
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
RT

Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation (2–4). These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,5). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (6).

Synthetic phosphopeptide corresponding to residues surrounding serine 10 of human histone H3.
Corresponding antibody, H5110-13J, detects endogenous histone H3 only when phosphorylated at serine 10. It is a useful tool in characterizing histone phosphorylation and in monitoring mitosis and meiosis with immunocytochemistry. Does not crossreact with other phosphorylated histones, or with histone H3 modified by acetylation. Recognizes human, mouse, rat, monkey.
Applications
Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified
Concentration
As reported
Form
Supplied as a liquid in 20mM potassium phosphate, pH 7.0, 50mM sodium chloride, 0.1mM EDTA, 1mg/ml BSA, 1% DMSO, 5% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
(1) Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67, 545–579. (2) Hansen, J.C. et al. (1998) Biochemistry 37, 17637–17641. (3) Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41–45. (4) Cheung, P. et al. (2000) Cell 103, 263–271. (5) Thorne, A.W. et al. (1990) Eur. J. Biochem. 193, 701–713. (6) Hendzel, M.J. et al. (1997) Chromosoma 106, 348–360
USBio References
No references available
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