IgG2a, Heavy Chain

Brand

MaxLight™

Conjugate

MaxLight™490

Specificity

Recognizes the mouse gamma 2a immunoglobulin heavy chain. Recognizes an allotypic determinant upon mouse IgG2a. Reacts with the IgHIa allotype (as expressed in BALB/c mice). Does not react with the IgHIb allotype (as expressed in C57/BL mice).

Source Antibody

mouse

Purity

Purified by affinity chromatography from tissue culture supernatant.

EU Commodity Code

30021010

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Notes

Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Applications

Suitable for use in FLISA. Other applications not tested.

Recommended Dilutions

FLISA: coating antibody. Optimal working dilutions to be determined by researcher.

Storage and Stability

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

References

1. Jarman, E., et al., Immunology 112: 631-642 (2004). 2. Bazin, H., (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids. 615-618. Pergamon Press, Oxford and New York. 3. Bazin, H., et al., J. Immunol. Methods 66: 261-269. (1984) 4. Bazin, H., et al., J. Immunol. Methods. 71: 9-16 (1984). 5. Querinjean, P., et al., Eur. J. Biochem. 31: 354-359 (1972). 6. Nagao, K., et al., Am. J. Respir. Cell. Mol. Biol. 29: 314-320 (2003). 7. Ramos, J.D.A., et al., Philippine Science Letters 2: 38-48 (2009). 8. Ormstad, H., et al., Toxicology 188: 309-318 (2003). 9. Ormstad, H., et al., J. Toxicol. Environ. Health A 61: 55-67 (2000). 10. Hall, G., et al., Vaccine 21: 549-561 (2003). 11. Tan, L.K., et al., Vaccine 24: 5762-5771 (2006). 12. Instanes, C. & Hetland, G., Toxicology 204: 13-21 (2004). 13. Lieden, A., et al., Allergy 64: 304-311 (2009). 14. Wolfowicz, C.B., et al., Vaccine 21: 1195-1204 (2003).

USBio References

No references available

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I1904-83A-ML490 IgG2a, Heavy Chain (MaxLight 490)

Brand

Conjugate

Specificity

Source Antibody

Purity

EU Commodity Code

Shipping Temp

Storage Temp

Notes

References

USBio References

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