Recognizes the rat IgG2b heavy chain. Does not crossreact with other immunoglobulin classes or subclasses.
Source Antibody
rat
Purity
Purified by affinity chromatography.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free BSA Free
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 5ug/ml (as a coating antibody) Optimal working dilutions to be determined by researcher.
Avidity for rat IgG2b: 4.7 x 10e9 M-1.
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
References
1. Sato, K., et al., Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat transplant. J. Immunol. 166: 4185-4194 (2001).