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I1906-04E-ML550 IgY, Heavy Chain (MaxLight 550)

Specifications
References
Brand
MaxLight™
Conjugate
MaxLight™550
Specificity
Recognizes both the IgY and IgY(-Fc) Peking duck heavy chain molecules. Species Crossreactivity: goose. Recognizes ~118kD and 178-200kD in pekin cell lysates under non-reducing conditions. Recognizes IgY heavy chains in Mallard duck, goose and swan.
Source Antibody
duck
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications
Suitable for use in Flow Cytometry, Western Blot and FLISA. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Western Blot: Non-reducing Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Magor, K.E. et al. (1994) One gene encodes the heavy chains for three different forms of IgY in the duck. J. Immunol. 153: 5549-5555.|2. Higgins, D.A. and Warr, G.W. (1993) Duck immunoglobulins: structure, functions and molecular genetics. Avian Pathol. 22: 211-236.
USBio References
No references available
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