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I7656-02 Insulin ELISA Kit, Human, BioAssay™

Specifications
Brand
BioAssay™
Kit Type
ELISA
Tests
96
Detection Method
Colorimetric/Fluorescent
Detection Range
Fasting: 5-35ulU/ml|30 minutes after oral glucose : 30-230ulU/ml|120 minutes after oral glucose: 14-160ulU/ml
Sample Matrix
Serum
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C

Kit Components
I7656-02A: Microtiter Plate, 1x96 wells I7656-02B: Standard A (0ulU/ml), 1x2ml I7656-02C: Standard B (6.25ulU/ml), 1x1ml I7656:02D: Standard C (12.5ulU/ml), 1x500ul I7656-02E: Standard D (25ulU/m)l, 1x500ul I7656-02F: Standard E (50ulU/ml), 1x500ul I7656-02G: Standard F (100ulU/ml), 1x500ul I7656-02H: Antibody (HRP), (20X), 1x1ml I7656-02J: Wash Buffer (20X), 1x25ml I7656-02K: HRP Substrate Solution, 1x12ml I7656-02L: Stop Solution, 1x12ml I7656-02M: Assay Diluent, 1x14ml
Storage and Stability
Store components at 4ºC. Stable for at 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Reference Range
Fasting: 5-35ulU/ml 30 minutes after oral glucose : 30-230ulU/ml 120 minutes after oral glucose: 14-160ulU/ml
Specificity
There is no crossreactivity with C-peptide at the concentration of 5000pmol/ml, with intact human proinsulin (biosynthetic) 0.3%. High concentrations of lipid or bilirubin do not interfere in the insulin assay. Purified hemoglobin up to 50ug/ml has been shown NOT to interfere the test. No interference for rheumatoid factor or human anti-mouse antibodies (HAMA) was observed.
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