Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. Lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 56 nm, is proportionate to the lactate concentration in the sample.
Applications
Direct Assays: Lactate in serum, plasma, and cell media samples.
Detection limit
Sensitive and accurate 96-well plate assay: 0.05mM and linearity up to 2mM L-Lactate. For cell culture samples containing phenol red: 0.1mM and linearity up to 1mM L-Lactate in 96-well plate assay.
Convenient: The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 minutes. Room temperature assay. No 37°C heater is needed.
High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Kit Components: (100 tests in 96-well plates)
L1011-04A1: Assay Buffer, 1x10ml L1011-04A2: NAD Solution, 1x1ml L1011-04A3: Enzyme A, 1x120ul L1011-04A4: Enzyme B, 1x120ul L1011-04A5: MTT Solution, 1x1.5ml L1011-04A6: Standard, 1x1ml Supplied in 20mM L-Lactate
Storage and Stability
Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Precautions
Reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.