Mucins are high molecular weight glycoproteins that are heavily glycosylated with oligosaccharide side chains linked O-glycosidically to the protein backbone. At least nine mucin genes have been identified to date. The pattern of tissue and cell-specific expression of these mucin genes suggests a distinct role for each member of the diverse mucin family. In epithelial cancers, many of the phenotypic markers for pre-malignant and malignant cells have been found on the carbohydrate and peptide moieties of mucin glycoproteins. Deregulation of tissue and cell-specific expression of mucin genes has also been described in epithelial cancers. At present, the role of mucin glyoproteins in various states of epithelial cell carcinogenesis, including the pre-neoplastic state and metastasis, is under investigation. The expression of MUC5AC has been frequently observed in gastric carcinomas and may be used as a marker of gastric differentiation.

Suitable for use in FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher.

Normal human epithelial tissues: bronchus, endocervix, stomach and gallbladder

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.