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N6925-ML650 Nuclei (MaxLight 650)

Specifications
References
Brand
MaxLight™
Conjugate
MaxLight™650
Specificity
Recognizes nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). Does not react with nuclei from mouse or rat. Other mammalian or animal species have not been tested for reactivity. Reacts with a ~80kD and ~70kD band in Immunoprecipitation.
Source Antibody
human
Purity
Purified by Protein A affinity chromatography.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Immunofluorescence, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Vescovi, et al., Experimental Neurology 156: 71-83 (1999). 2. Uchida, et al., Proc. Nat. Acad. Sci. USA 96: 14,720-14,725 (2000). 3. Kohama, I., et al., J. Neuroscience 21: 944-950 (2001). 4. Zhang, S.C., et al., Nature Bio. 19: 1129-1133 (2001). 5. Sigurjonsson, O., et al., Proc. Nat. Acad. Sci. USA 102(14): 5227-5232 (2005). 6. Klein, S.M., et al., Hum. Gene Ther. 16(4): 509-521 (2005).
USBio References
No references available
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