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R1035-15 Ral BP1, Recombinant, Rat (Ral Assay Reagent) (Agarose)

Specifications
References
Conjugate
Agarose
Purity
≥95%
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C

GST-tagged fusion protein corresponding to residues 397-519 of rat Ral binding protein, expressed in E. coli.

Applications
Affinity Precipitation: 10-15ul (5-7.5ug) of a 50% slurry precipitated RalAGTP and RalB-GTP from 1mg of EGF-stimulated Cos-7 cells which had been lysed in a magnesium containing lysis buffer. Note: Due to spontaneous deactivation of RalA and RalB, use lysates that have been stored at-70°C for less than 1 month.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Purity
≥95%
Concentration
As Reported
Form
Supplied as a liquid suspension, 50% agarose slurry in 50mM Tris, pH 7.5, 200mM sodium chloride, 1% NP40, 1mM PMSF, 1mM aprotinin prior to the addition of glycerol to 50%.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Goi, T., et al., Mol. Cell. Biol. 19: 1731–1741, 1999
USBio References
No references available
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