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R2031 Ribonuclease T1, Aspergillus oryzae (RNase T1) (300Ku/mg)

Specifications
References
Purity
Chromatographically purified (SDS-PAGE)
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C
EC=3.1.27.3; Guanyloribonuclease; Ribonucleate-3’-guanylo-oligonucleotidohydrolase

Ribonuclease T1 is an endonuclease which cleaves RNA or deaminated RNA between guanosine and inosine 3’-phosphate residues and the 5’-hydroxy residues of adjacent nucleotides with the formation of the corresponding intermediate 2’, 3’-cyclic phosphates.

Quality Control
Certified free of contaminating non-specific endo-nuclease, exo-nuclease and ribonuclease
Activity
≥300,000 units/mg protein
Protein Assay
As reported
Unit Definition
One unit releases the equivalent of one unit A260 of acid-soluble products from yeast RNA in 15 minutes at 37°C at pH 7.5.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at 4°C. Aliquots are stable for 6 months after recepit at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Aspergillus oryzae
Purity
Chromatographically purified (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 2.8M ammonium sulfate.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Egami, F., et al., Prog Nucleic Acid Res. 3: 59 (1964). 2. Fields, R., et al., J. Biochem. 121: 591 (1971).
USBio References
No references available
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