Mouse ascites obtained from mice bearing different hybridomas, delipidated, heat-inactivated, pooled and depleted of all immunoglobulins.
Applications
Suitable for use as a negative control for ELISA, Indirect Immunofluorescence and Western Blot, replacing the specific ascites in the test arrangement. Other applications have not been tested.
Recommended Dilutions
The control ascites is used in a dilution comparable to that of the specific ascites. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration
Not Determined
Form
Supplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.