Numerous protein and ribonucleoprotein components are required to catalyze pre-mRNA splicing. The SR proteins are essential splicing factors. They regulate alternative splicing of many pre-mRNAs. Alternative splicing is a major mechanism for controlling expression of cellular and viral genes. SR proteins are a family of highly conserved serine/arginine rich RNA-binding proteins. Nine human SR proteins including SF2/ASF are currently known. Based on primary sequence and on motifs shared with other proteins, human SF2/ASF is a 248aa protein composed of four domains. SF2/ASF exists predominantly or exclusively in a highly phosphorylated state in a majority of cells.

Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Calculated MW: 27.7kD Western Blot: ~33kD (Doublet or triplet bands sometimes evident due to phosphorylation differences).

HeLa cells

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.