Description In order to study the function of DNA, protein, RNA and their relationship, it is very important to get RNA, DNA and protein from the same piece of tissue. DNA, Protein, RNA Extraction Kit provides a convenient and efficiency system for isolation of RNA, DNA and protein from the same piece of tissue simultaneously. The isolated RNA can be used for mRNA isolation, probe generation, RT-PCR, Northern blot analysis, primer extension, RNA protection assay, and In vitro translation. The isolated DNA can be used for PCR amplification. And the isolated protein can be used for Western Analysis. The kit is enough for isolating DNA/RNA/Protein from 5 gram tissues or 10 gram cells or blood.

A representative kit from the same lot is randomly selected for isolation of RNA, DNA and protein. The quality and purity of isolated total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. Beta-actin gene, 838 bp fragment, is successfully amplified from the isolated genomic DNA. And the isolated protein’s quality is ensured by Western blotting analysis with anti-GAPDH antibody.

*If precipitate formed in solution 3, place the bottle at 65°C water bath to dissolve it before use. Item Amount Storage Part No. 1. Solution 1 50ml 4°C

2. Solution 2 6ml RT

3. Solution 3 50ml RT*

4. Phenol B 50ml 4°C

5. 0.5% SDS 25ml RT

6. DEPC H2O/0.1mM EDTA 50ml RT

7. TE buffer 5ml RT

8. RNAase (10ug/ul) 14ul -20°C