0.156-10ng/ml

<0.061ng/ml

Intra-Assay CV: <10% Inter-Assay CV: <12%

*027620A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027620B: Standard, 2x1vial 027620C: Standard Diluent, 1x20ml *027620D: Detection Reagent A (green), 1x120ul *027620E: Detection Reagent B (red), 1x120ul 027620F: Assay Diluent A, 1x12ml 027620G: Assay Diluent B, 1x12ml 027620H: TMB Substrate, 1x9ml *027620J: Positive Control, 0.94ng/ml, 1x1 vial (Lyophilized) 027620L: Wash Buffer, 30X, 1x20ml

Store *027620A, *027620B, *027620D, *027620E and *027620J at -20°C. After reconstitution of *027620J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.