The Glutathione Peroxidase 3 (GPX3) BioAssay™ ELISA Kit is to be used for the in vitro quantitative determination of human GPX3 in serum, plasma and cell culture supernatant. This ELISA Kit is for research use only.

This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human GPX3 in biological fluids. A polyclonal antibody specific for GPX3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, GPX3 is recognized by the addition of a biotinylated polyclonal antibody specific for GPX3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of GPX3 in the samples.

044865A: Microtiter Strips, 1x96wells (6x16 wells) 044865B: Wash Buffer, 10X, 2x30ml 044865C: ELISA Buffer, 10X, 2x30ml 044865D: Detection Antibody, 1x20ul 044865E: Streptavidin (HRP) (Lyophilized), 1x2ug *044865F: GPX3 Standard (Lyophilized), 1x1vial 044865G: TMB Substrate Solution, 1x12ml 044865H: Stop Solution, 1N Sulfuric Acid, 1x12ml

Store *044865F powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.