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045167 Progranulin BioAssay™ ELISA Kit (Human) (Proepithelin, PEPI, PC Cell-derived Growth Factor)

Specifications
References
Brand
BioAssay™
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C
Proepithelin, PEPI, PC Cell-derived Growth Factor

Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. New cutting-edge publications have shown that progranulin might be a biomarker not only for FTLD, but also for other types of Alzheimer's disease (AD). This new data even supposes the potential of progranulin to be a biomarker for MCI (Mild Cognitive Impairment), which would be a hallmark in AD research. Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.

The Progranulin (human) ELISA Kit is sandwich assay to be used for the in vitro quantitative determination of human progranulin in serum, plasma, urine, cell culture supernatant and cerebrospinal fluid (CSF; R. Ghidoni et al.; Neurology 71, 1235 (2008)).
Detection Range
0.063-4ng/ml
Sensitivity
32pg/ml
Test Principle
A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples.
Kit Components
Coated plate with human progranulin Antibody , 1x96wells Wash Buffer 10X, 2x30ml ELISA Buffer 10X, 2x30ml Detection Antibody, 1x30ul HRP Labeled Streptavidin (lyophilized), 1x2ug human progranulin Standard (lyophilized), 1x8ng TMB Substrate Solution, 1x12ml Stop Solution, 1x12ml
Storage and Stability
Store kit at 4°C, stable for 12 months after receipt. After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use.
References
Product Reference: |●Low plasma progranulin levels predict progranulin mutations in frontotemporal lobar degeneration: R. Ghidoni, et al.; Neurology 71, 1235 (2008)|●Common variation in the miR-659 binding-site of GRN is a major risk factor for TDP43-positive frontotemporal dementia: R. Rademakers, et al.; Hum. Mol. Genet. 17, 3631 (2008)|●Serum progranulin concentrations may be associated with macrophage Infiltration into omental adipose tissue: B.S. Youn, et al.; Diabetes 58, 627 (2009)|●Plasma progranulin levels predict progranulin mutation status in frontotemporal dementia patients and asymptomatic family members: N. Finch, et al.; Brain 132, 583 (2009)|●Progranulin plasma levels as potential biomarker for the identification of GRN deletion carriers. A case with atypical onset as clinical amnestic Mild Cognitive Impairment converted to Alzheimer's disease: M. Carecchio, et al.; J. Neurol. Sci. 287, 291 (2009)|●Cerebrospinal fluid progranulin levels in patients with different multiple sclerosis subtypes: M. De Riz, et al.; Neurosci. Lett. 469, 234 (2010)|●Low Serum Progranulin Predicts the Presence of Mutations: A Prospective Study: E.C. Schofield, et al.; J. Alzheimers Dis. 22, 981 (2010)|●A Novel Progranulin Mutation Causing Frontotemporal Lobar Degeneration with Heterogeneous Phenotypic Expression: G. Rossi, et al.; J. Alzheimers Dis. 23, 7 (2010)|●Pathogenic cysteine mutations affect progranulin function and production of mature granulins: J. Wang, et al.; J. Neurochem. 112, 1305 (2010)|●Microglial upregulation of progranulin as a marker of motor neuron degeneration: T. Philips, et al.; J. Neuropathol. Exp. Neurol. 69, 1191 (2010)|●A novel progranulin mutation causing frontotemporal lobar degeneration with heterogeneous phenotypic expression: G. Rossi, et al.; J. Alzheimers Dis. 23, 7 (2011)|●rs5848 polymorphism and serum progranulin level: G.Y. Hsiung, et al.; J. Neurol. Sci. 300, 28 (2011)|●Association of TMEM106B Gene Polymorphism With Age at Onset in Granulin Mutation Carriers and Plasma Granulin Protein Levels: C. Cruchaga, et al.; Arch. Neurol. 68, 581 2011|●Progranulin expression in brain tissue and cerebrospinal fluid levels in multiple sclerosis: M. Vercellino, et al.; Mult. Scler. 17, 1194 (2011)|●Soluble Beta-amyloid precursor protein is related to disease progression in amyotrophic lateral sclerosis: Steinacker, et al.; PLoS One. 6, e23600 (2011)|●Loss of function mutations in the progranulin gene are related to pro-inflammatory cytokine dysregulation in frontotemporal lobar degeneration patients: P. Bossù, et al.; J. Neuroinflammation 8, 65 (2011)|●Mucosal Progranulin expression is induced by H. pylori, but independent of Secretory Leukocyte Protease Inhibitor (SLPI) expression: T. Wex, et al.; BMC Gastroenterol. 11, 63 (2011)|●Progranulin Genetic Screening in Frontotemporal Lobar Degeneration Patients From Central Italy: S. Bagnoli, et al.; Cell Mol. Neurobiol. 32, 13 (2011)|●Optimal Plasma Progranulin Cutoff Value for Predicting Null Progranulin Mutations in Neurodegenerative Diseases: A Multicenter Italian Study: R. Ghidoni, et al.; Neurodegener. Dis. (Epub ahead of print) (2011)|●Prevalence of frontotemporal lobar degeneration in an isolated population: the Vallecamonica study: N. Gilberti, et al.; Neurol. Sci. (Epub ahead of print) 2011|●Granulin mutation drives brain damage and reorganization from preclinical to symptomatic FTLD: B. Borroni, et al.; Neurobiol. Aging. (Epub ahead of print) 2011|●Mining the Gastric Cancer Secretome: Identification of GRN as a Potential Diagnostic Marker for Early Gastric Cancer: H. Loei, et al.; J. Proteome Res. (Epub ahead of print) 2012|●
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