Hyaluronan and proteoglycan link protein 1, CRT1, CRTL1, HPLN1, Cartilage-linking protein 1, Proteoglycan link protein
HAPLN1, also known as CRTL1, is a member of the hyaladherin family. This protein contains a common structural domain of about 100aa that is termed a Link module with two alpha-helices and two antiparallel beta-sheets. It involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. It stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Also, It has been shown to interact with Versican, two extracellular matrix components essential for cardiac development. Recombinant human HAPLN1, fused to His-Tag at C-terminal, was expressed in HEK293 and purified by using conventional chromatography techniques.
Source
Recombinant protein corresponding to aa16-354 from human HAPLN1, fused to His-Tag at C-terminal, expressed in HEK293.
Molecular Weight
~39.3kD (345aa)
Amino Acid Sequence
DHLSDNYTLD HDRAIHIQAE NGPHLLVEAE QAKVFSHRGG NVTLPCKFYR DPTAFGSGIH KIRIKWTKLT SDYLKEVDVF VSMGYHKKTY GGYQGRVFLK GGSDSDASLV ITDLTLEDYG RYKCEVIEGL EDDTVVVALD LQGVVFPYFP RLGRYNLNFH EAQQACLDQD AVIASFDQLY DAWRGGLDWC NAGWLSDGSV QYPITKPREP CGGQNTVPGV RNYGFWDKDK SRYDVFCFTS NFNGRFYYLI HPTKLTYDEA VQACLNDGAQ IAKVGQIFAA WKILGYDRCD AGWLADGSVR YPISRPRRRC SPTEAAVRFV GFPDKKHKLY GVYCFRAYN<H HHHHH>
Biological Activity
Measured by its binding ability in a functional ELISA with Hyaluronic acid. The ED50 range ≤ 1ug/ml.
Applications
Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, HEK293 cells
Form
Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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