Recognizes an unidentified antigen in the cytoplasm of mat ure granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of m yeloid cells are useful in the identification of different levels of cellular differentiation. Monoclonal antibody BM-2 reacts with early precursor and mature forms of human myeloid cells. This monoclonal antibody is useful for the detection o f myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.

Suitable for use in FLISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher.

Tonsil or lymph node

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.