It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Applications:  Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot. Other applications not tested.

Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. Optimal dilutions to be determined by the researcher.

Biotinylated proteins in solution or on tissues

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.