This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGFa (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml FLISA: Coating antibody Immunoprecipitation: 1-2ug/500ug protein Immunohistochemistry (Frozen/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.

Jurkat or Ramos cells, heart, kidney, pituitary, Breast cancer, melanoma

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.