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214464-ML650 Actin, beta (ACTB and Beta Actin) (MaxLight 650)

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Conjugate
MaxLight™650
Specificity
Recognizes human Actin Beta. Species Crossreactivity: Mouse, porcine, rabbit and rat. Highly specific for cytoplasmic actin beta and does not react with other actin isoforms.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals. (Vandekerckhove et al. 1978).
Mouse anti Human actin beta, has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodelling (Miazza et al. 2011)
Applications
Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Dugina, V. et al. (2009) Beta and gamma­cytoplasmic actins display distinct distribution and functional diversity. J. Cell Sci. 155: 2980­2988 2. Brockmann, C. et al. (2011) Beta­ and gamma­cytoplasmic actins are required for meiosis in mouse oocytes. Biol Reprod. 85: 1025­39. 3. Miazza, V. et al. (2011) Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production. Virology. 410: 7­16. 4. Baranwal, S. et al. (2012) Nonredundant roles of cytoplasmic b­ and y­actin isoforms in regulation of epithelial apical junctions. Mol Biol Cell. 23: 3542­53. 5. Shagieva, G.S. et al. (2012) Actin Isoforms and Reorganization of Adhesion Junctions in Epithelial­Mesenchymal Transition of Cervical Carcinoma Cells Biochemistry (Moscow) BM12­154 6. Perrin, B.J. and Ervasti, J.M. (2010) The Actin Gene Family: Function Follows Isoform Cytoskeleton (Hoboken). 67: 630­4.
USBio References
No references available
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