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214684-ML490 CD4 (MaxLight 490)

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Conjugate
MaxLight™490
Specificity
Recognizes bovine CD4. Species Crossreactivity: Bison
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Recognizes a 50kD transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Suitable for use on formal dichromate (FD5) fixed paraffin embedded tissues with amplification and antigen retrieval techniques Optimal dilutions to be determined by the researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Bensaid, A. et al. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4). Vet. Immunol. Immunopathol. 27: 51-54. 2. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets. Vet. Immunol. Immunopathol. 27: 215-231. 3. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 4. Winkler, M. et al (1999) Bovine Herpesvirus 1 Can Infect CD4+ T Lymphocytes and Induce Programmed Cell Death during Acute Infection of Cattle. J Virol. 73:8657–8668. 5. Winkler, M. et al. (2000) Persistence and Reactivation of Bovine Herpesvirus 1 in the Tonsils of Latently Infected Calves. J Virol. 74: 5337–5346. 6. Riondato, F. et al. (2008) Effects of road transportation on lymphocyte subsets in calves Vet J. 175: 364-8. 7. Collins, R.A. et al. (1999) Bovine interleukin-12 and modulation of IFNgamma production. Vet Immunol Immunopathol. 68: 193-207. 8. Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas. Vet Pathol. 44: 366-72. 9. Sühwold, A. et al. (2010) T cell reactions of Eimeria bovis primary and challenge-infected calves. Parasitol Res. 106: 595-605. 10. Rhodes, S.G. et al. (2000) Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease. Immunology. 99: 195-202. 11. Machugh, N.D. et al. (1997) Identification of two distinct subsets of bovine gamma delta T cells with unique cell surface phenotype and tissue distribution. Immunology. 92: 340-5. 12. Childerstone, A.J. et al. (1999) Demonstration of bovine CD8+ T-cell responses to foot-and-mouth disease virus. J Gen Virol. 80: 663-9. 13. Rhodes, S.G. et al. (2001) Antigen recognition and immunomodulation by gamma delta T cells in bovine tuberculosis. J Immunol. 166: 5604-10. 14. Knowles, G. et al. (1996) Phenotypical characterization of lymphocytes infiltrating regressing papillomas. J Virol. 70: 8451-8. 15. Smyth, A.J. et al. (2001) In vitro responsiveness of gammadelta T cells from Mycobacterium bovis-infected cattle to mycobacterial antigens: predominant involvement of WC1(+) cells. Infect Immun. 69: 89-96. 16. Hein, W.R. and Dudler, L. (1997) TCR gamma delta+ cells are prominent in normal bovine skin and express a diverse repertoire of antigen receptors. Immunology. 91: 58-64. 17. Juleff, N. et al. (2009) Foot-and-mouth disease virus can induce a specific and rapid CD4+ T-cell-independent neutralizing and isotype class-switched antibody response in naïve cattle. J Virol. 83: 3626-36. 18. Hope, J.C. et al. (2005) Exposure to Mycobacterium avium induces low-level protection from Mycobacterium bovis infection but compromises diagnosis of disease in cattle. Clin Exp Immunol. 141: 432-9. 19. Sopp, P. et al. (2006) Flow cytometric detection of gamma interferon can effectively discriminate Mycobacterium bovis BCG-vaccinated cattle from M. bovis-infected cattle. Clin Vaccine Immunol. 13: 1343-8. 20. Sacchini, F. et al. (2011) A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. mycoides. Vet Res. 42: 77. 21. Skyberg, J.A. et al. (2011) Murine and bovine ?d T cells enhance innate immunity against Brucella abortus infections. PLoS One. 6(7): e21978. 22. Cantón, G.J. et al. (2013) Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation. Vet Res. 44: 60.
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