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214685-ML550 CD4 (MaxLight 550)

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Conjugate
MaxLight™550
Specificity
Recognizes canine CD4.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992]. Rat anti canine CD4 has been demonstrated to partially deplete circulating T lymphocytes when administered in vivo, but alone was not sufficient to prolong allograft survival in a canine transplant model (Watson et al. 1993).
Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocytes subsets Moore et al. 1992. Rat anti Canine CD4 forms part of a panel of anti canine monoclonal antibodies used extensively in the evaluation of leukemic status in dogs (Villiers 2002).
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens 43(3): 137-154. 2. Gorman, S. et al. (1994) Isolation and expression of cDNA encoding the canine CD4 and CD8a antigens.Tissue Antigens 43(3): 184-188. 3. Watson, C.J.E. et al. (1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog. Brit. J. Surg. 80: 1389-1392. 4. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.Can J Vet Res. 73: 137-143. 5. Bauer. T.R. Jr. et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.Blood. 108: 3313-20. 6. Boggiatto, P.M. et al. (2010) Immunologic indicators of clinical progression during canine Leishmania infantum infection.Clin Vaccine Immunol. 17: 267-73. 7. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75. 8. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.J Nutr. 133: 1158-62. 9. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.Cell Immunol. 263: 88-98. 10. Estrela-Lima, A. et al. (2010) Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates.BMC Cancer. 10: 256. 11. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.Immunology. 105: 499-508. 12. Pinheiro, D. (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog.Immunology. 132: 111-22. 13. Mitchell, L. et al. (2012) Clinical and immunomodulatory effects of toceranib combined with low-dose cyclophosphamide in dogs with cancer.J Vet Intern Med. 26: 355-62. 14. Tominaga, M. et al. (2010) Flow cytometric analysis of peripheral blood and tumor-infiltrating regulatory T cells in dogs with oral malignant melanoma.J Vet Diagn Invest. 22: 438-41.
USBio References
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