≥20,000units/ug protein

One unit is defined as the amount of enzyme required to incorporate 1pmol phosphate into Kemptide substrate per min at 30°C.

The activity of the PKA catalytic subunit is assayed in 40mM Tris-HCl, 20mM magnesium acetate, 200uM [gamma-32P]ATP (500-1000cpm/pmol) with 130uM Kemptide substrate in a total volume of 60ul. The reaction is incubated at 30°C for 5min and terminated by spotting 40ul of the reaction mix onto P-81 filters and washing in 0.5% H3PO4 for 5 min for a total of 4 washes. Following the final wash, the filters are rinsed in ethanol, dried, and counted in the presence of scintillation fluid. A value of 200pmol obtained for Kemptide phosphorylation under these conditions corresponds to 1pmol phosphorylated casein.

Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bovine Heart

≥90% (SDS-PAGE)

As reported

Supplied as a liquid in 350mM K2HPO4, pH 6.8, 100uM DTT.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.