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227417-ML650 CD4 (MaxLight 650)

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
Purity
Purified by Protein A affinity chromatography form tissue culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Conjugate
MaxLight™650
Specificity
Recognizes equine CD4.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

CD4 is a 58kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells.
A study undertaken using 227417, to identify CD4 on several wild african equid species, indicates that CD4 recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Lunn, D.P.et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunol. 74: 251-257. 2. Kydd, J.H. and Antczak, D.F., (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991 Equine Immunol. 4-5. 3. Deeg,C.A. et al. (2004) The uveitogenic potential of retinal S-antigen in horses. Invest Ophthalmol Vis Sci. 45: 2286-92. 4. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 71: 213-7. 5. Brault, S.A. et al. (2010) The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness. Vet Immunol Immunopathol. 137: 136-41. 6. Goodman, L.B. et al. (2007) A point mutation in a herpesvirus polymerase determines neuropathogenicity. PLoS Pathog. 3(11):e160. 7. Hamza, E. et al. (2011) CD4(+)CD25(+) T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity. Vet Immunol Immunopathol. Jun 6. [Epub ahead of print]. 8. Go, Y.Y. et al. (2010) Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes. J Virol. 84: 4898-911. 9. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62: 101-143. 10. Ibrahim, S. (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28. Anchor Text.
USBio References
No references available
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