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299085 Hoagland’s No. 2 Basal Salt Mixture (Powder)

Specifications
References
Grade
Plant Grade
EU Commodity Code
38210000
UN DOT Shipping
UN1486 PGIII
Shipping Temp
Blue Ice
Storage Temp
4°C

Hoagland’s No. 2 Basal Salt Mixture is a classic plant medium. Hoagland’s solution is a popular solution for a wide variety of plant species.

Suitability
Plant cell culture tested
Formula variant
With the macro- and micronutrients as described by Hoagland and Arnon (1938).
Appearance
Grayish-purple, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
pH
As Reported
Directions per Liter
Dissolve 1.63 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at 4ºC. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Formulationmg/L
Ammonium Phosphate Monobasic115.03
Boric Acid2.86
Calcium Nitrate656.4
Cupric Sulfate•5H2O0.08
Ferric Tartrate•2H2O5.32
Magnesium Sulfate240.76
Manganese Chloride•4H2O1.81
Molybdenum Trioxide0.016 
Potassium Nitrate606.6
Zinc Sulfate•7H2O0.22
Total:1.63g/L
References
1. The water-culture method for growing plants without soil. Hoagland, D.R., and Arnon, D.I. Univ. Calif. Coll. Agric. Exp. Sta. Circ. Berkeley, CA , 347-353, (1938)
USBio References
No references available
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