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299712 Murashige and Skoog Modified Basal Salt Mixture (Powder)

Specifications
References
Grade
Plant Grade
EU Commodity Code
38210000
UN DOT Shipping
UN1486 PGIII
Shipping Temp
Blue Ice
Storage Temp
-20°C/4°C

Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exception: no ammonium nitrate.

Appearance
Off white to tan, homogenous, free flowing powder
Solubility
Colorless to pale yellow, clear, complete
pH
As Reported
Directions per Liter
Dissolve 2.68 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at -20°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Media Formulation
Components shown as mg/L
Boric Acid
Calcium Chloride
Cobalt Chloride•6H2O 0.025
Cupric Sulfate•5H2O 0.025
EDTA Disodium•2H2O 37.26
Ferrous Sulfate•7H2O 27.8
Magnesium Sulfate
Manganese Sulfate•H2O 16.9
Potassium Iodide 0.83
Potassium Nitrate1900
Potassium Phosphate, Monobasic 170
Molybdic Acid (Sodium Salt)•2H2O 0.25
Zinc Sulfate•7H2O 8.6
Total
References
1. Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
USBio References
No references available
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