Schenk and Hildebrandt Vitamin

Grade

Plant Grade

EU Commodity Code

38210000

Shipping Temp

Blue Ice

Storage Temp

4°C

Contains the vitamins as described by Schenk and Hildebrandt (1972).

Appearance

Off-white to white, homogenous, free flowing powder

Solubility (1X)

Colorless, clear, complete

pH (1X)

As Reported

Directions per Liter

Dissolve 101.05g in 800-900ml of ddH2O stirring gently until completely solubilized. Add sodium bicarbonate if needed. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at 4ºC. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as mg/L

myo-Inositol 100,000
Nicotinic Acid (Free Acid) 500
Pyridoxine•HCl 50
Thiamine•HCl 500

Total:

101.05g/L

References

1. Schenk, RU and AC Hildebrandt. 1972. Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Can. J. Bot. 50: 199-204.