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A3578-FITC ART2.2 (ADP Ribosyltransferase 2.2) (FITC)

Specifications
References
Grade
Affinity Purified
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Conjugate
FITC
Specificity
Recognizes the gene product of the mouse ADP-ribosyltransferase (ART) 2.2 gene, an ortholog of rat RT6.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

ART2.2 is expressed exclusively by mature T-cells. Levels of ART2.2 expression vary between inbred strains of mice and is absent in NZW mice, in which the ART2.2 gene is deleted (1).

Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized wistar rats were fused with cells of the Sp2/0 mouse myeloma cell line.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Koch-Nolte, F. et al. (1999). A new monoclonal antibody detects a developmentally regulated mouse Ecto-ADP-Ribosyltransferase on T cells: subset distribution, inbred strain variation and modulation upon T cell activation. Journal of Immunolo
USBio References
No references available
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