Bile Esculin Agar is prepared according to the formula specified by Swan, and later evaluated by Facklam and Moody. It was concluded that the bile esculin test provided a reliable means of identifying enterococci and differentiation from non-enterococci. Bile Esculin Azide Agar is a more selective medium as described by lsenberg. Enterococci are bile tolerant and hydrolyze esculin which imparts a dark brown color to the medium. Sodium azide inhibits Gram negative and Gram positive bacteria other than enterococci are inhibited by the bile salts.

Beige, homogeneous. free flowing powder

Grayish yellow, opalescent/trace to light haze

7.1 ± 0.2

Note: Growth results obtained from type cultures after incubation at 35°C and observed after 18-24 hours.

Escherichia coli (ATCC 25922): Inhibited Entertococcus fecalis (ATCC 29212): Good Growth, Blackening of the medium

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Dissolve 56.25 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.