In 1926 Wilson and Blair used Bismuth and Sodium Sulfite in a culture formulation for the isolation of typhoid-paratyphoid groups of bacteria bacteria. Further modifications of the formula were described by Hajna and Damon. The current recommended formulation is found in the USP. Bismuth and brilliant green dye acts as inhibitors to most enteric bacteria and Gram positive bacteria. Salmonella typhi produces black colonies surrounded by a metallic sheen. Other Salmonella produce green or black colonies. Some Shigella sp. may grow on this medium.

Light beige, homogeneous and free flowing

Greenish-yellow and opaque.

7.5± 0.2

Escherichia coli (ATCC 25922): Partial Inhibition Brown to Green Enterococcus faecalis (ATCC 29212): Inhibited Salmonella typhi (ATCC 19430): Growth Black with metallic sheen Salmonella typhimurium (ATCC 14028): Good Black with metallic sheen

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Dissolve 52g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.