These Blood Base media contain highly nutritious extracts or digests of beef. The beef products supply vitamins, carbohydrates, salts and other organic nitrogen compounds. Other peptones provide additional growth factors. Sodium chloride provides essential ions and electrolyte balance.

Escherichia coli (ATCC 25922): Growth, Beta hemolysis Staphylococcus aureus (ATCC 25923): Growth, Beta hemolysis Streptococcus pneumonia (ATCC 6305): Growth, Alpha hemolysis Streptococcus pyogenes (ATCC 19615): Growth, Beta hemolysis

*Results were obtained from type cultures after incubation at 35°C and observed after 18-24 hours. Media prepared with 5-10% sheep blood.

Beige, homogeneous and free flowing powder

Red and opaque

7.4 ± 0.2

Dissolve 40 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15 psi). Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium. Cool to 45-50°C. Dispense into Petri dishes or tubes.

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.