Brain Heart Infusion (BHI) Agar has proved to be effective in the cultivation of a wide variety of microorganisms, including many types of pathogens. BHI Agar is currently recommended as a general medium for aerobic bacteria and for the primary recovery of fungi from clinical specimens. BHI Agar, with 10% Sheep Blood can be used to isolate systemic fungi that may grow poorly on nonenriched media. A high recovery rate for Histoplasma capsulatum can be obtained with the addition of horse blood to the medium.

Beige homogeneous, free flowing powder

Trace to slight haze, light to medium amber

7.4±0.2

Aspergillus brasiliensis ATCC 16404: Growth Candida albicans ATCC 10231: Growth Escherichia coli ATCC 25922: Growth Neisseria meningitides ATCC 13090: Growth Saccharomyces cerevisiae ATCC 9763: Growth Staphylococcus aureus ATCC 25923: Growth Streptococcus pneumoniae ATCC 6305: Growth Streptococcus pyogenes ATCC19615: Growth

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Dissolve 52 grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.