Brucella Agar is a prepared medium for the cultivation of Brucella sp. from clinical and dairy products. With the addition of blood and menadione, Brucella Agar can be used to cultivate anaerobic bacteria. Cultivation of Brucella sp. requires CO2 enriched

Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.

Brucella abortus ATCC 4315: Growth Brucella melitensis ATCC 4309: Growth Brucella suis ATCC 4314: Growth Escherichia coli ATCC 25922: Growth Streptococcus pyogenes ATCC 19615: Growth

Light beige, homogeneous, free flowing powder.

Yellow beige, slightty turbid.

7.0±0.2

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter Suspend 43 grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). To prepare blood plates, cool medium to 45°- 50.0°C and add aseptically 5% sheep red blood or 10% horse blood. Dispense into sterile Petri plates.

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.