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C2383-02D-ML650 CD31 (MaxLight 650)

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Conjugate
MaxLight™650
Specificity
Recognizes sheep CD31, also known as PECAM-1|Species Crossreactivity: bovine and goat
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells. Reported to inhibit homotypic leukocyte aggregation induced by anti CD43 antibodies (2). Removal of sodium azide is recommended prior to use in fuctional assays.
Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Optimal dilution determined by the researcher.
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells of the SP2-0/Ag14 mouse myeloma cell line.
Recommended Negative Control
I1904-83P1: IgG2a Murine Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Brodersen, R. et al. (1998). Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species: Vet. Immunol. Immunopathol. 64: 1-13. 2. Pintado. C. O. et al. (1995). A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti CD43 monoclonal antibodies of ruminant leukocytes. Immunol. Lett. 45: 81-85.
USBio References
No references available
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    C2383-02D-ML650 Mab
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