Centrimide Agar is based on Tech Agar, which was developed by King, Ward and Raney. Centrimide is prepared according to this formula with the addition of Centrimide (cetyltrimethylammonium bromide). Cetrimide is a quaternary ammonium, cationic detergent with limited disinfectant action. It causes nitrogen and phosphorus to be released from the bacterial ceII. This germicidal activity is effective on most microorganisms except Pseudomonas aeruginosa. Centrimide Agar Base is supplemented with 1% Glycerol as a source of carbon.

Beige, homogeneous, free flowing powder

Light to medium beige, slightly turbid

7.2±0.2

Escherichia coli ATCC 8739: Inhibited Pseudomonas aeruginosa ATCC 27853: Growth, yellow-green colonies Pseudomonas aeruginosa ATCC 10145: Growth, yellow-green colonies Pseudomonas aeruginosa ATCC 9027: Growth, yellow-green colonies Staphylococcus aurous ATCC 25923: Inhibited

Dissolve 46.7g per liter of ddH2O, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Cool to 45.0°C and pour into sterile Petri dishes.

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.