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C3999 Chick Embryo Extract, Ultrafiltrate, Liquid (CEE)

Specifications
References
Grade
Molecular Biology Grade
Form
Pale-yellow, clear, solution
EU Commodity Code
38210000
Shipping Temp
Blue Ice
Storage Temp
4°C

Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an ultrafiltration step with a 10kD MW cutoff to remove protein from the solution, producing a clear, amber liquid. This liquid is sterile-filtered.

Animals are sourced from a specific USDA-Registered facility using a veterinary-inspected flock. Final processing at an ISO9000 facility. Chick Embryo Extract, Ultrafiltrate is protein free. It does not support the growth of viruses.
Appearance
Pale-yellow, clear, solution
Total Protein (Biuret)
~0.0g/dL
Sterility (per 9CFR 113.26)
Negative
Mycoplasma (per 9CFR 113.26)
Negative
Osmolality
As Reported
Microbial Testing
Pullorum typhoid, Galisepticum, M. synoviae, M. meleagridis): Negative
Recommended Dilutions
1ml of extract/100ml of media
Storage and Stability
Chick Embryo Extract, Ultrafiltrate is protein-free; thus, it will not break down. May be stored at RT. Stable for 12 months after receipt. Storage at 4°C extends the stability for an additional 3-6 months. Some precipitate has been observed when frozen.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
Source
11-14 day old chick embryos
Form
Pale-yellow, clear, solution
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
US Biological Application References: 1. Griffin, M.A., et al., J. Cell Science 117: 5855-5863 (2004). 2. Clause, K.C., et al., Tissue Engineering Part C 16: 375-385 (2010). 3. Montemurro, T., et al., J. Cell Mol. Med. 15(4): 796–808 (2011). 4. Jiang, X., et al., Stem Cells Dev. 18: 1059-1070 (2009). 5. Lokireddy, S., et al., Am. J. Physiol. Cell Physiol. (2011) http://ajpcell.physiology.org/content/early/2011/09/01/ajpcell.00114.2011. 6. de la Garza-Rodea, A.S. et al., (2013) FASEB J. doi: 10.1096/fj.13-233155. 7. Park S., et al. 2014. J Exp Zool Mol Dev Evol. 322:156-165. 8. O'Connell G.C. and Pistilli E.E. Biochem Biophys Res Commun. 2015. 458:614-619. 9. Escobar H, et al. Molecular Therapy—Nucleic Acids (2016) 5, e277; doi:10.1038/mtna.2015.52. 10. Masuda S, et al. 2018. Acta Physiol (Oxf). 222(3). doi: 10.1111/apha.12975. Epub 2017 Oct 19. 11. Ahrens HE, et al. 2018. Skelet Muscle. 8(1):20. doi: 10.1186/s13395-018-0166-x.
USBio References
1. Griffin, M.A. et al., (2004) J. Cell Science 117:5855-5863. |2. Clause, K.C. et al., (2010) Tissue Engineering Part C, 16:375-385. |3. Montemurro, T. et al., (2009) J Cell Mol Med. Mar 9, Epub. |4. Jiang, X. et al., (2009) Stem Cells Dev. 18:1059-1070. |5. Lokireddy, S. et al., (2011) Am J Physiol Cell Physiol, http://ajpcell.physiology.org/content/early/2011/09/01/ajpcell.00114.2011 OR http://www.ncbi.nlm.nih.gov/pubmed/21900687. |6. de la Garza-Rodea, A.S. et al., (2013) FASEB J. doi:|10.1096/fj.13-233155. |7. Park S., et al. 2014. Conserved regulation of hoxc11 by pitx1 in Anolis lizards. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 322: 156-165. |8. O'Connell G.C. and Pistilli E.E. Interleukin-15 directly stimulates pro-oxidative gene expression in skeletal muscle in-vitro via a mechanism that requires interleukin-15 receptor alpha. Biochem Biophys Res Commun. 2015. 458(3):614-619.|9. Escobar H, et al. Full-length Dysferlin Transfer by the Hyperactive Sleeping Beauty Transposase Restores Dysferlin-deficient Muscle. Molecular Therapy—Nucleic Acids (2016) 5, e277; doi:10.1038/mtna.2015.52.|10. Masuda S, et al. Chemokine (C-X-C motif) ligand 1 is a myokine induced by palmitate and is required for myogenesis in mouse satellite cells. 2018. Acta Physiol (Oxf). 222(3). doi: 10.1111/apha.12975. Epub 2017 Oct 19.|11. Stilhano RS, et al. Reduction in skeletal muscle fibrosis of spontaneously hypertensive rats after laceration by microRNA targeting angiotensin II receptor. 2017. PLoS One. 12(10):e0186719. doi: 10.1371/journal.pone.0186719.|12. Novak JS, et al. 2017. Myoblasts and macrophages are required for therapeutic morpholino antisense oligonucleotide delivery to dystrophic muscle. Nat Commun. 8(1):941. doi: 10.1038/s41467-017-00924-7.|13. Ahrens HE, et al. Klotho expression is a prerequisite for proper muscle stem cell function and regeneraton of skeletal muscle. 2018. Skelet Muscle. 8(1):20. doi: 10.1186/s13395-018-0166-x.|14. Nihashi Y., et al. 2019. Toll-like receptor ligand-dependent inflammatory responses in chick skeletal muscle myoblasts. Dev Comp Immunol. 91:115-122. doi: 10.1016/j.dci.2018.10.013. Epub 2018 Oct 30.|15. Akpulat U., et al. 2018. Shorter Phosphorodiamidate Morpholino Splice-Switching Oligonucleotides May Increase Exon-Skipping Efficacy in DMD. Mol Ther Nucleic Acids. 13:534-542. doi: 10.1016/j.omtn.2018.10.002.
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