For isolation of Clostridium difficile. Clostridium Difficile Agar is a formula recommended by George et al. for isolation of C. difficile. The medium contains fructose which enhances the recovery and growth of C. difficile. The addition of the antibiotics, cefoxitin and cycloserine, inhibit the growth of Enterobacteriaceae, Enterococcus faecalis, Staphylococci, Gram negative non-sporulating anaerobic bacilli, and Clostridia sp. but not C. difficile. Supplementing the medium with horse blood and egg yolk further improves the recovery of C. difficile.
Microbiological Analysis
Bacteroides fragilis ATCC 8482: Inhibited Clostridium difficile ATCC 9689: Good Growth Clostridium perfringens ATCC 13124: Inhibited Staphylococcus aureus ATCC 25923: Inhibited
*Results were obtained from type cultures after incubation at 35°C in anaerobic conditions and observed at 48-72 hours. Media was prepared with 7% horse blood, 0.5g/L Clycloserine and 0.016g/L Cefoxitin
Appearance
Light beige, homogeneous, free flowing powder
Solubility
Light amber, clear to slightly hazy
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 69.1grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Cool to 45-50°C and add 7% of horse blood and Cycloserine (0.5g/L) and Cefoxitin (0.016 g/L). Alternately, neutral red (0.03g/L) may be added prior to autoclaving and Cycloserine and Cefoxitin added after autoclaving.
Storage and Stability
Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Components shown as g/liter |
| Proteose Peptone | 40.0 |
| Fructose | 6.0 |
| Monopotassium Phosphate | 1.0 |
| Disodium Phosphate | 5.0 |
| Magnesium Sulfate | 0.1 |
| Sodium Chloride | 2.0 |
| Agar | 15.0 |
TDS
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