Suitable for the cultivation of anaerobes and stock culture maintenance. Used to differentiate saccharolytic from proteolytic Clostridium sp. Saccharolytic species form acid and gas without digesting the meat. Proteolytic species form sulfur compounds (putrid smell) and break down the meat to amino acids (blackening). Also suitable for recovery of Clostridium botulinum from foods.

Expected cultural response after 40-48 hours at 35°C.

Yellowish brown to brown granules

Medium amber supernatant, insoluble granules

7.6-8.0

Dissolve 11.54grams per 100ml of distilled/deionized (DDI) water Mix thoughly and allow to stand for 15 minutes until all the particles are thoroughly wetted. If required add 0.2grams glucose. Mix well and while maintaining an even suspension, distribute into test tubes. Autoclave for 15 minutes at 121ºC (15psi).

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.